Preference for near-cognate codons in translation initiation. (A) Heatmaps showing the percent frequencies of each type of codon among the identified TISs (left) and among annotated CDSs of all genes (middle), and the TIS codon enrichment (log2 ratio between the codon abundance among identified TISs and that among annotated CDSs; right). Enrichment values are shown for AUG and individual near-cognate codons in tomato, Arabidopsis, and humans. (B) The median translation initiation efficiency (TE) of TISs at AUG and individual near-cognate codons identified in tomato (orange) and humans (blue) (Methods). (C) The correlation between TIS codon enrichment and tRNA abundance in tomato. (r) Pearson correlation coefficient.
