CRISPR-based modular assembly of a UAS-cDNA/ORF plasmid library for more than 5500 Drosophila genes conserved in humans

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Figure 4.
Figure 4.

The UAS-cDNA/ORF constructs generated by CRISPRmass were functional in the presence of GAL4 in Drosophila. UAS-CG7747, UAS-Prdm13, and 38 UAS-DUB transgenes were screened for phenotypes by crossing to eye-specific GMR-GAL4 (A), wing-specific A9-GAL4 (B), and muscle-specific MEF2-GAL4 (C) drivers, respectively. Activation of the UAS-Prdm13 and several UAS-DUB transgenes elicited distinct phenotypes (arrows).

This Article

  1. Published in Advance November 13, 2019, doi: 10.1101/gr.250811.119 Genome Res. 30: 95-106

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