CRISPR-based modular assembly of a UAS-cDNA/ORF plasmid library for more than 5500 Drosophila genes conserved in humans

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Figure 2.
Figure 2.

Restriction analysis of 40 UAS-cDNA/ORF constructs generated by CRISPRmass. UAS-CG7747, UAS-Prdm13, and 38 UAS-DUB constructs were generated by CRISPRmass from the DGRC Gold Collection and analyzed by PstI or PvuII digestion, and the expected restriction patterns for all the UAS-cDNA/ORF constructs were observed (Supplemental Table S1). (M) DNA marker.

This Article

  1. Published in Advance November 13, 2019, doi: 10.1101/gr.250811.119 Genome Res. 30: 95-106

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