Sequence error correction (ec) and analysis of cross-contamination using error-corrected SNVs. (A) Comparison of error rates between random sampling and ec in trinucleotide context. The number in each box indicates the error rate and is colored by its intensity. The middle base in the trinucleotide context is the “source” base, and the single base on top of each column is the “destination” base. For each “destination” base, the first column corresponds to random sampling method, and the second column corresponds to the ec method. (B) Minority SNV ratios of SK-BR-3 nuclei and SKN1 nuclei from the four-nuclei mixing experiment using the ec method showing very low contaminations between BAGs.
