Snhg14 lncRNA activates stem cell core factor gene promoters. (A) LncRNA expression vectors. Snhg14 was expressed under the control of the CMV promoter. RsRed fluorescence marker was used as the expression control (Ctl), and the empty vector was used as the vector control (Vector). The expression vectors were cotransfected with stem cell core factor gene promoter-luciferase reporter vector in 293T cells and luciferase activity was measured. (B) Snhg14 activates the Pou5f1 promoter. (pPou5f1) Pou5f1 promoter; (Luci) luciferase; (pA) SV40 poly(A) signal. A 4-kb Pou5f1 promoter DNA fragment was cloned in front of luciferase. 293T cells were cotransfected with Snhg14 expression vector and the pPou5f1-luciferase vector DNAs using Lipofectamine 2000. Forty-eight hours after transfection, luciferase activity was measured using a Promega luciferase assay kit. The activity of luciferase was adjusted by using the Vector control as 1. (**) P < 0.01 as compared with the Ctl and Vector controls. (C) The activity of the Sox2-luciferase. The Snhg14 expression vector DNA was cotransfected with the pSox2-luciferase vector DNA in 293T cells. (NS) No statistical significance as compared with the Ctl and Vector controls. (D) Effect of Snhg14 on the activity of the Nanog promoter. The Snhg14 expression vector DNA was cotransfected with the pNanog-luciferase vector DNA in 293T cells. (*) P < 0.05, (**) P < 0.01 as compared with the Ctl and Vector controls.
