Profiling the long noncoding RNA interaction network in the regulatory elements of target genes by chromatin in situ reverse transcription sequencing

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Figure 4.
Figure 4.

Knockdown of Sox2-interacting lncRNAs down-regulates stemness genes. (A) Lentiviral shRNA knockdown vectors. (pU6) RNA polymerase III U6 promoter; (pCMV) CMV promoter; (CopGFP) fluorescence tracking marker; (shRNA) shRNAs targeting Snhg14 and Platr10; (shCT) shRNA random control. Lentiviruses were packaged in 293T cells and were used to infect iPSCs. (B) Knockdown of Snhg14 and Platr10 lncRNAs in iPSCs. After lentiviral transfection, the shRNA-expressing iPSCs were selected by puromycin and were collected for quantitative PCR. (**) P < 0.01 as compared with shCT and vector controls. (C) Down-regulation of three core stem cell factor genes in Snhg14-knocking down iPSCs. (**) P < 0.01 as compared with shCT and vector controls.

This Article

  1. Published in Advance July 17, 2019, doi: 10.1101/gr.244996.118 Genome Res. 29: 1521-1532

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