Epididymal adipose tissue targeting of (dCas9/sgFabp4) + ATS-9R oligoplexes. (A) Biodistribution image of intraperitoneally injected (dCas9/sgFabp4) + ATS-9R oligoplexes at 12 and 24 h postinjection. (B) Mean fluorescence intensity in various organs. Adipocytes were differentiated into mature adipocytes for in vitro analysis. For in vivo studies, 6-wk-old male C57BL/6 mice were fed on standard chow or a high-fat diet for 11 wk. Total RNAs were extracted and relative mRNA levels were determined by real-time PCR. Fabp4 mRNA was increased in the mature adipocytes (C) as well as both in the epidydimal white adipose tissue (epiWAT) and subcutaneous white adipose tissue (subWAT) of obese mice (D). Phb mRNA levels also increased in (E) mature adipocytes and (F) obese epiWAT. Data are presented as mean ± SEM. (n = 4–5). (ns) nonsignificant, (**) P < 0.01, (***) P < 0.001 was considered significant.
