D538G mutant affects migration but not proliferation in endometrial cancer cells. (A) Bar graphs indicating the doubling times for ESR1 wild-type and D538G mutant cell lines in full media and hormone-deprived (HD) media. The relative wound densities of two ESR1 wild-type and three D538G mutant cell lines are shown over 24 h following scratch/wounding of cell monolayer in full serum media (B) and HD media (D). Images show the initial wound (pink) and migratory cells (orange) in wild-type and D538G mutant cells at 0 and 24 h in full serum media (C) and HD media (E). Proliferation and migration figures represent at least three independent experiments, performed in triplicate. Error bars represent SEM.
