Figure 2.
D538G mutant drives a distinct transcriptional program. (A) Heatmap shows the relative expression of E2 up- and down-regulated genes as well as mutant-specific differentially expressed genes. Sample types are indicated by the column annotations described in the legend. Validation of RNA-seq results by qPCR are shown for ligand-independent E2–up-regulated genes PGR (B) and MMP17 (C) as well as mutant-specific up-regulated gene EHF (D) and down-regulated gene EPHA3 (E). B–E show average expression levels, normalized to wild-type cells without E2 treatment, for two ESR1 wild-type and three D538G mutant clones after 8-h E2 or DMSO (vehicle) induction. Error bars represent SEM.
