Performance evaluation of MeSMLR-seq on bulk-level nucleosome occupancy and single-molecule long-range phasing of nucleosomes. (A) Correlation of nucleosome occupancy profiles generated by MeSMLR-seq and MNase-seq. For MeSMLR-seq, the molecules that were aligned to forward (fwd) and reverse (rev) genomic strands were analyzed separately. (B) Nucleosome occupancy profiles at the bulk-cell level provided by MeSMLR-seq and MNase-seq. (C) Overlap of nucleosomes detected by MeSMLR-seq and MNase-seq at the bulk-cell level. (D) Number of nucleosomes phased at single sequencing molecules of MeSMLR-seq data under 2% glucose condition. (E) Detection and phasing of nucleosomes at the single-molecule level by NP-SMLR. Each gray line represents a molecule. Green oval represents nucleosome.
