CD276 is an immune gene with elevated expression in GSCs. (A) 3D genome and chromatin landscape at the CD276 locus. This panel integrates CTCF ChIP-seq, H3K27ac ChIP-seq, RNA-seq, compartments (50-kb), domains (10-kb), and loops (union of 5-, 10-, and 25-kb calls) determined by Hi-C for three patient-derived GSC cultures (G523, G567, and G583). (B) Expression of CD276 was determined by RNA-seq in bulk GBM samples (n = 76), GSCs (n = 76), and non-neoplastic brain tissue (n = 4). P-values were calculated with the Mann–Whitney U statistical test. (C) Survival of glioma patients stratified by CD276 expression in the French data set. Median gene expression was used to stratify patients. P-value was derived with log-rank statistics. Shading around curve indicates 95% confidence interval. (D) Patterns of expression of CD276 in the prenatal and postnatal human brain. Data were extracted from BrainSpan.
