Figure 5.
Introns retained in polyadenylated zygotic transcripts. (A) Analysis of intron retention within transcripts of genes activated during the NC 9–10. For each intron with >20 informative reads, the fraction retained was calculated (Katz et al. 2010), and the number of introns falling within each bin of retention were plotted. A value of 0.0 describes a completely spliced intron and a value of 1.0 reflects a 100% retained intron. (B) As in A but analyzing zygotically transcribed RNA isolated at the syncytial-blastoderm stage. (C) As in A but analyzing zygotically transcribed RNA isolated at the cellularized-blastoderm stage.
