Transcription factor association with nascent and mature chromatin. (A) Heatmap of small DNA-binding factor occupancy in mature and nascent chromatin (log2 [mature/nascent]) for 6272 high-confidence sites obtained from DNA fragment lengths between 20 bp and 80 bp. (B) Enrichment of small DNA fragments (DNA-binding factors) for the three classes of chromatin maturation: fast, equal occupancy in nascent and mature chromatin; slow, greater occupancy in mature than nascent chromatin; and transient, greater in nascent than mature. (C) Average plot of nucleosome organization at sites occupied by the factors described in B. (D) Sites of transient occupancy are promiscuously enriched at sites distal from the transcription start site (TSS) (P < 2.2 × 10−16 between fast and transiently associating factors).
