Genome-wide reconstitution of chromatin transactions reveals that RSC preferentially disrupts H2AZ-containing nucleosomes

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Figure 6.
Figure 6.

Reconstituted H2AZ-containing nucleosome arrays are preferential RSC targets as well. (A) Schematic of the principle of the nucleosome array ejection assay, with supercoiled plasmid (topoisomer) distribution revealed by 2D gel. (Lk) Linking number, (N) nicked, (L) linear. RSC-dependent changes in this assay are ATP-dependent (Clapier et al. 2016). (B) Nucleosome arrays assembled with canonical octamers (WT Array) or with H2AZ-containing octamers (H2AZ Array), incubated +/– RSC. A representative replicate is shown.

This Article

  1. Published in Advance May 16, 2019, doi: 10.1101/gr.243139.118 Genome Res. 29: 988-998

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