RSC prefers H2AZ-containing nucleosomes. (A) H2AZ-containing nucleosomes in the 99th RS percentile and their position around the TSS (red), compared with pattern for all nucleosomes in same (black). (B) H2AZ as in A, but in different RS percentiles. (C) Time-course of RSC remodeling preference with nucleosomes from wild type (left) and htz1Δ, (right) by qPCR. Blue bars show average of four strongly remodeled, H2AZ + TSS nucleosomes, and orange bars show average of four control nucleosomes (see Supplemental Fig. S5D,E). Asterisks show statistical significance; (*) P < 0.05, (**) P < 0.01; Student's t-test. (D) Position around the TSS of nucleosomes in the different RS percentiles, for wild-type (WT) and htz1Δ nucleosomes, respectively. (E) Comparison of the IS and RS for H2AZ+ and H2AZ− around the TSS of genes. For ease of comparison, the genomic mean of each score was set to zero by subtracting the genome-wide mean from each base pair.
