Turnover of ribosome-associated transcripts from de novo ORFs produces gene-like characteristics available for de novo gene emergence in wild yeast populations

A continuous emergence of putative polypeptides in S. paradoxus. Normalized RPF read coverage for a selection of lineage-specific (or group-specific) tORFs per strain. RPF read coverages are displayed for replicate 1 and 2 with a blue or pink area, respectively. The positions of all iORFs (including ntORFs and tORFs) in the genomic area are drawn below each plot. The tORF of interest is labeled with a yellow dot and is plotted in black. iORFs overlapping the iORF of interest are plotted in black when they are in the same reading frame and in gray when they are in a different reading frame than the selected tORF.