Early shift from inflammatory to pro-resolving monocytes/macrophages in chronic infection to prevent fibrosis. (A) Percentage of IL6-producing ITGAM+ ITGAX− LY6G− (also known as CD11b+ CD11c− LY6G−) cells and number of LY6C high monocytes/macrophages per spleen at day 6 postinfection. (B) Heatmap illustrating the relative expression of genes in monocyte/macrophages from acute and chronic infected mice at day 6 PI. (C) Representative images of Masson's trichrome staining of spleens. Thickness of the splenic capsules are marked by arrows. Magnification bar: 50 µm. (D) Semiquantification of fibrosis in spleens. (A–D) Data shown are the mean ± SEM of n = 4 to 6 mice. (*) P ≤ 0.05; (**) P ≤ 0.01; (***) P ≤ 0.001 unpaired two-tailed t-test.
