Changes in GC content between rapidly evolving mouse and human 3′ UTR sequences determine mRNA stability in fast-UTR reporter assay. (A) Example alignments (left) of rapidly evolving mouse GCLiPP peaks and human syntenic regions showing higher (Chd7), similar (Med24), or lower (B4galt5) GC content in the mouse sequence, with individual sequence fast-UTR measurements shown to the right. Correlation between steady-state mRNA abundance for rapidly evolving mouse GCLiPP peaks and corresponding human syntenic regions in mouse T cell fast-UTR assay (B) and difference in GC content between rapidly evolving mouse GCLiPP peaks and syntenic human regions (C) and ratio of fast-UTR steady-state mRNA abundance for the same. (r) Pearson correlation coefficient.
