Pyramidal neuron subclustering. (A) Subclustering of the NR1- and NR2-assigned cells using cisTopic and UMAP. (B) Cluster assignments identified using PhenoGraph. CA1 and CA3 neuronal populations exhibited strong signal at cell-type–specific marker genes. Asterisk indicates putative assignment based on modest enrichment at marker genes. Right panels show the NEUROD1 motif enrichment in the original t-SNE coordinates (top) that correspond to the region of cells assigned to CA3 cluster (bottom). (C) Biclustering of cisTopic topics and weights for each cell. Highlighted topics exhibit high cluster specificity. (D) Further subclustering of the Other/MC cell population produced three distinct groups, including putative mossy cells (MCs). (E) Biclustering of cisTopic topics and weights for each cell. Highlighted topics exhibit high cluster specificity. (F) Topic 5, specific to one of the subclusters, is highly enriched for AP-1–related motifs, suggesting the cells may be in a state of heightened activity.
