Figure 5.
SMC1 facilitates Rad21 and SA association with enhancers and origin-proximal promoters in BG3 cells. (A) SMC1 ChIP-seq of SMC1-depleted (iSMC1) cells. The violin plot distributions, promoter dot plots, and meta-analysis of early DNA replication origins show globally reduced SMC1 occupancy leaving similar residual levels at all features. (B) Effects of SMC1 depletion on Rad21 localization. (C) Effects of SMC1 depletion on SA localization. (D) Effects of SMC1 depletion on Nipped-B localization. All occupancy changes are statistically significant (Supplemental Table S2). ChIP-seq track examples are in Supplemental Figure S4.
