Loss-of-function mutations of cardiac TFs alters regulatory networks involved in heart development. (A) LSFM images of GFP-labeled CMs of wild-type and TF mutant zebrafish hearts at 72 hpf. The dotted line indicates exact area of the LSFM image. (B) Venn diagrams and GO enrichment analysis of TF mutant down-regulated (blue) and up-regulated (red) genes and chromatin accessibility of proximal promoter NFRs (±3 kb of TSS), adjusted P-value ≤ 0.05. (C) Percent distribution of cardiac module down-regulated genes/proximal NFR chromatin accessibility as compared to total number of TF mutants down-regulated genes/proximal NFR chromatin accessibility. (D) Percent distribution of cardiac module up-regulated genes/proximal NFR chromatin accessibility as compared to total number of TF mutants up-regulated genes/proximal NFR chromatin accessibility. (E) Cardiac module genes with differentially regulated expression and chromatin accessibility of proximal promoter NFRs (±3 kb of TSS) in gata5, hand2, and tbx5a mutants.
