Figure 1.
Schematic overview of identifying circular RNA (circRNA) regions. Assuming a circRNA molecule is present, a sequence read crossing the junction (green arrow) and its read-mate (gold arrow) would map to a linear reference in the manner depicted. The junction read would get multiple alignments, and the read-mate would be located in between the position of the junction read. Multiple read-pairs at the same junction strengthen the support for the circRNA. Subsequent additional filtering (details are in the Methods section) and annotation produced the list of circRNA regions.
