MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network

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Figure 6.
Figure 6.

PRC2 regulates FRMD4B expression through a feed-forward regulatory network with let-7i. (A) Genome Browser view of FRMD4B showing tracks for EZH2 and H3K27me3 ChIP-seq. (B) qRT-PCR showing expression changes of FRMD4B in miRNA-depleted (+ Dox) and nondepleted (− Dox) WT and EZH2−/− cells, plotted as in Figure 1E. (*) P < 0.05. (C) Genome Browser view of let-7i in two replicates of WT and EZH2−/− cells. (D) Luciferase assay comparing miRNA inhibition between WT and mutant FRMD4B 3′ UTR (seed deletion) transfected WT and EZH2−/− cells. (E) Feed-forward regulatory network formed by PRC2, LIN28B, let-7i, and FRMD4B.

This Article

  1. Published in Advance January 16, 2019, doi: 10.1101/gr.238311.118 Genome Res. 29: 184-192

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