Figure 6.
Experimental validation of isoforms predicted by AIDE and Cufflinks. Isoforms of genes MTHFD2 (A), NPC2 (B), RBM7 (C), CD164 (D), FGFR1 (E), and ZFAND5 (F) were validated by PCR and Sanger sequencing. The isoforms to validate (yellow) are listed under each gene (dark gray), with +/− indicating whether an isoform was/was not identified by PCR or a computational method. The forward (F) and reverse (R) primers are marked on top of each gene. For each gene, the agarose gel electrophoresis results show the molecular lengths of PCR products.
