Figure 5.
Independent confirmation of CNAs in original unamplified DNA by MLPA. Four recurrent loci with copy gain in M23 and M25 spanning genes with immune function (Supplemental Fig. S5) were tested, along with DNA from three mice that did not have the CNA (M6-L and -G, control L and 1-G, and control 2-G). (A) The height of the MLPA peak at each locus for each mouse is shown in proportion to the reference probe. Only the four loci in the T cell (but not the tail representing the germline) DNA from mouse M23 show an increase over 1. (B) Representative peaks shown as a percentage of the reference probe. Only mouse M23 shows doubling of the peak in T cells.
