Genes encoding the same protein complex are replicated simultaneously in HeLa cells. (A) Two protein complexes exemplify the synchronized replication of genes encoding the same protein complex. The standard deviation (sd) of replication timing within a protein complex is shown on the right. (B) The observed median standard deviation of replication timing within a protein complex is significantly smaller than the random expectation in which the protein-complex–coding genes were shuffled. (C) The comparison of the similarity in replication timing between the S-phase–expressed and –unexpressed protein complexes. The expressed protein complexes are defined as the complexes with at least two-thirds of subunits expressed; the expressed subunits are defined as the subunits expressed in at least half of the S-phase cells (CPM > 0). Outliers are omitted in the box plot. P-value was given by the Mann–Whitney U test.
