The functions of individual snRNAs are similar in basal and alternative splicing. (A) Two-dimensional clustering (multidimensional scaling) of samples based on transcriptome-wide splicing of different categories of splicing events, as in Figure 2B. The clustering was based on the events with the largest degree of variation across samples, as indicated by N. Red indicates U1; green, U2; blue, U4; purple, U6. (B,C) Overlap between exons (B) and introns (C) that are differentially spliced following KD of each snRNA in MCF-7 cells. Most events are unique to depletion of individual snRNA. (D) Enrichment of Gene Ontology (GO) biological process categories for genes containing differentially spliced exons (D) or introns (E). The displayed terms were selected based on their combined significance across the four samples. Only terms with at least two ancestors are illustrated. Circle size is proportional to the statistical significance.
