GG-NER complex chromatin occupancy and nucleosome remodeling after UV irradiation requires the ATPase function of Rad16. (A) Chromatin occupancy of the Rad16 ATPase mutated protein at GCBSs is plotted here using ChIP-chip data (Yu et al. 2016). The pre-UV irradiation data are shown by the black trace, while the 15-min post-UV chromatin occupancy is presented by the gray trace. The nucleosome landscape in the ATPase mutant is shown by the gray shading at these of positions. (B) MNase-seq data from the RAD16 ATPase mutant cells are shown at GCBS-associated ORFs in a 2-kbp window. The arrows on the x-axis indicate the relative orientation of the GCBSs and ORFs. The insets highlight the UV-induced changes to the nucleosome occupancy at the positions immediately adjacent to the GG-NER complex–occupied promoter.
