Figure 1.
Hyperosmotic stress has a strong impact on A/B compartment organization. (A) Representative immunofluorescence images of no stress or 1 h NaCl-treated cells for lamin B1 and H3K9me3, as well as stained with DAPI. (B) Correlation of eigenvalues obtained from Hi-C data for Chromosome 6 (Chr 6), comparing the two nonstressed replicates (R1 and R2) and comparing the nonstressed (R1) with the NaCl-treated (1 h 110 mM NaCl; R1) cells. (C,D) Eigenvalues and Hi-C maps from different regions of Chromosome 6 of nonstressed or NaCl-treated cells. (E) Contact probability of pairs of 100-kb-bin-dependent genomic distance on Chromosome 6, with or without NaCl treatment.
