Whole-genome bisulfite sequencing with improved accuracy and cost

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Figure 1.
Figure 1.

Overview of the BS-tagging assay. Standard transposases are used but with a 5mC fill-in. The bisulfite treatment changes the original transposon sequence and prevents it from being amplified, but the fill-in complement with 5mC remains amplifiable. One custom oligonucleotide is needed for this assay.

This Article

  1. Published in Advance August 9, 2018, doi: 10.1101/gr.232587.117 Genome Res. 28: 1364-1371

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