Dicer-like (DCL) gene family members and their expression in Asparagus, daylily and Lilium. (A) Heat map representing the expression profile of Asparagus, daylily, and Lilium AGO members. A phylogeny of AGO members is provided in Supplemental Figure S13. (B) Heat map of DCL abundances for three monocots for Dicer-like transcripts that were reliably detected (>1 FPKM) in at least one of three anther stages or the vegetative material. A phylogeny of DCL members is also provided in Supplemental Figure S13. (C) FISH localizing DCL5 transcripts in the cytoplasmic area of the tapetum and archesporial cells in meiotic-stage anthers from Lilium. The green channel (AF647) indicates DCL5 mRNA localization. DAPI (pink) shows the stained nucleus. In the middle image, the locations of the endothecium (ed), tapetum (ta), and archesporial cells (ar) are indicated. (Scale bar) 20 µm for all images.
