Synergistic effect of activation of viral defense pathway by dual depletion. (A) The expression levels of “defense response to virus” (GO:0051607) and “type I interferon signaling pathway” (GO:0060337) genes in Gene Ontology. The 87 genes were up-regulated at least in one sample more than twofold relative to nontarget siRNA. (B) Expression of ERV-Fc2 and DDX58 by RT-qPCR. Error bars represent SEM from three independent biological replicates. P-values were calculated using the two-tailed Student's t-test for comparisons with 5-aza-CdR plus siNontarget samples. (C) Gene Ontology analysis of biological processes enriched for up-regulated genes by dual depletion. The x-axis indicates Bonferroni corrected −log2 P-values in each dual depletion sample.
