Expression of BELD genes show vulnerability to disruption of ASD-associated chromatin regulators. (A) Boxplots of expression changes of non-BELD control genes and BELD genes in response to MECP2 R106W mutation (mouse cortex), Kdm6b knockdown (cortical neurons), or TOP1 inhibition by topotecan (cortical neurons). P indicates P-value, one-tailed t-test. (B) Boxplots of expression changes of non-BELD control genes and BELD genes in response to Chd8 knockdown in cortical neurons or Kdm5c knockout in cortical tissues. P indicates P-value, one-tailed t-test. (C) Boxplots of H3K4me1 changes in gene body regions of non-BELD control genes and BELD genes in the cortex of Kdm5c knockout mice. P indicates P-value, one-tailed t-test. (D) Diagram of genomic profiling and siRNA knockdown in Neuro 2a cells. (E,F) The expression changes of two BELD genes and two non-BELD genes in response to Kmt2c knockdown (E) or to Kdm6b knockdown (F) in Neuro 2a cells. (***) P < 3.76 × 10−7, one-tailed t-test; (*) P < 8.81 × 10−3, one-tailed t-test.
