Characterization of maize and sorghum isoforms from PacBio Iso-Seq. (A) Comparison of transcript length distributions between maize reference annotations and Iso-Seq isoforms. (B) Comparison of transcripts length distributions between sorghum reference annotations and Iso-Seq isoforms. (C) Classification of Iso-Seq isoforms of maize and sorghum. (D) Distribution of splicing patterns across tissues in maize. (E) Distribution of splicing patterns across tissues in sorghum. (F) Heatmap of PSI values across tissues in maize. (G) Heatmap of PSI values across tissues in sorghum. (ES) Exon skipping; (A5) alternative 5′ splice-site; (A3) alternative 3′ splice-site; (IR) intron retention; (AF) alternative first exon; (AL) alternative last exon.
