Arrayed CRISPR screen with image-based assay reliably uncovers host genes required for coxsackievirus infection

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Figure 5.
Figure 5.

HeLa cells were transiently transfected with siRNAs and sgRNAs targeting ACBD3 or CSDE1. (A) Cells were infected with CVB3, fixed, and stained. The percentage of CVB3-infected cells (normalized to sgRNA or siRNA control). Mean ± SEM for triplicate experiments. (**) P < 0.001, (n.s.) nonsignificant. (B,C) Cells were transfected with control siRNA, ACBD3 siRNA, and CSDE1 siRNA. The expression level of each protein was measured by western blot using the anti-ACBD3 antibody (B) and anti-CSDE1 antibody (C).

This Article

  1. Genome Res. 28: 859-868

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