Transmission of satellite-free centromeric domains in hybrids. (A) Family trees reporting the genetic relationships among the individuals used in this study. Each color represents an individual, and the same color code is used in B. Cell lines from the individuals in gray were not available (NA). (B) ChIP-seq analysis performed with the anti-CENPA antibody on chromatin from the DonkeyB cell line and the cell lines from its offspring MuleA, MuleB, and MuleC. For each cell line, the results of three experiments are shown. The centromeres of donkey Chromosomes 4 (EAS4) and 7 (EAS7) are shown as examples, and the other centromeres are reported in Supplemental Figure S7. The EquCabAsiB chimeric genome was used as reference.
