HNRNPA1 overexpression correlates with global redistribution of U2AF2 signal to Alu RNA elements. (A) Aggregated crosslinking sites on antisense Alu elements and nearby regions for U2AF2. Blue represents wild-type binding of the given RNA binding protein, and red represents HNRNPA1 overexpression of the log10 number of iCLIP read counts across all antisense-Alu elements. (B) Aggregated crosslinking sites on sense Alu elements and nearby regions for U2AF2. (C) Scatter plot of all human cassette exons measuring the proportion of U2AF2 iCLIP crosslinking sites found within Alu elements within the cassette exon event over the total number of crosslinks found within the event. Proportions from control and HNRNPA1 overexpression samples are compared for each individual cassette exon event. (D) Box plot representing the distance of Alu elements from cassette exons (blue) and constitutive exons (orange) that show no change in U2AF2 crosslinking versus those that show an increase in U2AF2 crosslinking.
