Meiotic heterochromatin is enriched for H3K9me2 and shows increased MLH1 foci in kyp suvh5 suvh6. (A) Wild-type (Col) male meiocytes immunostained for ASY1 (red) and H3K9me2 (green) and stained for DNA (DAPI, blue), at leptotene, zygotene, and pachytene stages. Scale bar, 10 µM. (B) As for A, but showing leptotene stage cells from wild type (Col), cmt3-11, and kyp suvh5 suvh6. (C) Male meiocytes at diakinesis stained for DAPI and immunostained for MLH1 (green) in wild type and kyp suvh5 suvh6. Bivalent chromosomes are evident that are associated with MLH1 foci at crossover sites. (D) Quantification of H3K9me2 immunostaining in wild type (Col), cmt3, and kyp suvh5 suvh6 meiotic cells. (E) Quantification of MLH1 foci in wild type and kyp suvh5 suvh6.
