Analyses of experimental data sets validate miRNA target site function. Responses to transfection (A–C) or knockout (D) of indicated miRNAs in human (A–C) or mouse (D) cell types. Each panel depicts corresponding changes in mRNA levels (A,B), in fraction of Argonaute-bound genes (C), and in mRNA stability and translational efficiency as measured by ribosome protected fragments (RPF; D). In each case, X-linked genes and the human orthologs of Z-linked genes containing target sites with an assigned PCT score (red) for the indicated miRNA were compared with all expressed genes lacking target sites (black); gene numbers are indicated in parentheses. (A,B,D) (***) P < 0.001, two-sided Kolmogorov–Smirnov test. (C) (*) P < 0.05, two-sided Fisher's exact test.
