Delineation of an exogenous plasmid DNA array integration and duplication event. (A) Dot plot of the ruIs32 insertion in contig1884 against the pAZ132 plasmid and unc-119 gene (red) that were integrated by biolistic transformation. The y-axis shows the pAZ132 plasmid structure and the unc-119 gene structure. The insertion contains three copies of GFP::H2B and two partial copies of unc-119. (B) MinION read coverage for Chromosome III. Note the ∼2-Mb duplication in the region where ruIs32 has been integrated. Red line is coverage and purple shading is coverage deviation. (C) MinION-generated sequence identified the two wild-type copies of unc-119 inserted with ruIs32 and the unc-119(ed3) mutation in contig0079. Superscript numbers refer to position within the contig.
