Transcription rate strongly affects splicing fidelity and cotranscriptionality in budding yeast

Distribution of the novel splice sites in RP (purple) and non-RP (orange) intron-containing transcripts whose SEF is significantly different in the fast and slow mutants relative to WT (P < 0.01). (A,B) Alternative 5′SS and 3′SS splicing events whose fidelity increased with the fast mutant. (C,D) Alternative 5′SS and 3′SS splicing events whose fidelity reduced with the fast mutant. (E,F) Alternative 5′SS and 3′SS splicing events whose fidelity increased with the slow mutant. (G,H) Alternative 5′SS and 3′SS splicing events whose fidelity reduced with the slow mutant. Green region upstream of 3′SS is the mean distance between BP and annotated 3′SS in budding yeast (∼37 nt).