Figure 2.
Depletion of HNRNPU increases lamina-chromatin association. (A) Smoothed Lamin B1 DamID signals show different distributions in the control (shCtrl) and Hnrnpu knockdown (shU). (B) Lamin B1 (LB1) DamID signal tracks across one 8.2-Mb region on Chromosome 15. Black bars represent locations of LADs, and regions changed from non-LADs to LADs were highlighted in light blue. (C) Detailed changes of LADs with Hnrnpu knockdown. (D) Box plots showing gene expression changes in regions gained or lost of LADs. P-values were obtained by Wilcoxon rank-sum test. (E) Electron microscopy analysis of AML12 cells treated with different shRNAs. Scale bar, 2 µm.
