Figure 2.
Effects of chromatin structure on Cas9 activity at off-target sites. (A) Mismatched sgRNAs that differed from the Cas9 target sequence by 1 or 2 nt were targeted to sites in both open (blue) and closed (red) chromatin regions in HEK 293T and HeLa cells. Indel frequencies were measured using targeted deep sequencing. Error bars, SEM (n = 3). (B) Indel frequencies at the on-target sites in open and closed chromatin states using on-/off-target specific sgRNAs in HeLa cells. Indel frequencies were measured using targeted deep sequencing. Error bars, SEM (n = 3).
