Extension of silent domains predicts major subtelomeric chromatin transitions. (A) Pearson's correlation matrix between Sir3 binding and histone marks, SIR3 oe corresponds to yAT1254 and SIR2 and SIR3 oe to yAT1668. Histone modification data from Weiner et al. (2015) for all marks except H3K79me2 (Schulze et al. 2009). (B) Genome browser visualization of Sir3 binding in WT, pGPD-SIR2 pGPD-SIR3 strains, in G1-blocked cells, in H3Δ4-30 mutants and selected histone modifications (from Weiner et al. 2015) in WT strains at TELVIR. (C) Distribution of selected histone marks relative to H3 (data from Weiner et al. 2015) along wild-type silenced domains and within the contiguous subtelomeric domains accessible to Sir3 upon overexpression. For comparison, the distributions of those marks within the 5 kb contiguous to the end of extended silent domains as well as within euchromatin (i.e., ESD excluded, n = 49,313) are shown.
