Effects of multiple enhancers on target gene expression and chromosomal conformation. (A) Schematic diagram of TNFSF10 gene with its multiple enhancers. TNFSF10 has #38 enhancer 27 kb upstream, and #30 and #5 enhancers 67 and 69 kb downstream, respectively. Colored arrows from the gene and enhancer indicate transcriptional direction. Dashed lines show physical interaction between promoter and enhancer (Interaction A and B) or between different enhancers (Interaction C). (B) Interaction changes between enhancer and promoter regions (Interaction A and B) after individual eRNA KD and all three combined eRNA KD. (C) Three eRNAs (dark blue) and target gene (orange) expression fold changes after each individual eRNA KD and after all three eRNA KD by combining three corresponding siRNAs.
