Open chromatin is permissive to advancement but not delay of replication timing. (A) Heatscatter plot of the H3K9R/HWT ratio of RT values (log2 S/G1) versus the H3K9R/HWT ratio of FAIRE signal at all 10-kb windows across the major chromosome scaffolds; 10-kb windows with significantly advanced (red) or delayed (blue) RT are indicated. Darker color indicates higher density of windows. (B) Cumulative count of advanced (red) or delayed (blue) 10-kb windows ordered by increasing FAIRE signal in H3K9R compared to HWT. (C) Heatscatter plot of the H3K9R/HWT ratio of HP1a ChIP signal versus the H3K9R/HWT ratio of FAIRE signal at all 10-kb windows across the major chromosome scaffolds. (D) Venn diagram of all 10-kb windows with significantly altered FAIRE or HP1a signal in H3K9R compared to HWT (P < 0.01; edgeR). For all panels, significantly different RT was determined as P < 0.05, log2 fold change > 0.1 using limma.
