Technical comparison of 250, 100, and 25 ng of DNA sequenced with both standard-DS and CRISPR-DS. Measurements were obtained by sequencing samples prepared with standard-DS (blue) using one and two rounds of hybridization capture and CRISPR-DS (red) with only one round of hybridization capture. (A) The percentage of raw sequencing reads on-target (covering TP53) post-capture(s) was comparable between standard-DS with two rounds of capture and CRISPR-DS with one round of capture, demonstrating the target enrichment efficiency of the novel method. (B) Percentage recovery was calculated as the percentage of genomes in input DNA that produced DCS reads. CRISPR-DS increases recovery thanks to the initial CRISPR-based target enrichment, which eliminates one round of hybridization capture. (C) After creating DCS reads, the median DCS depth across all targeted regions was calculated for each input amount. The increased recovery enabled by CRISPR-DS translates into five to ten times more sequencing depth for the same input DNA.
