Two differential cellular states of EPI cells defined by the lncRNAs and co-expression networks. PCA (A) and hierarchical clustering (B) analyses based on lncRNA expression profiles separated the EPI cells of EB and MB stages from those of LB and HB stages. (C) Differentially expressed lncRNAs in EPI cells of four blastocyst stages (FDR < 0.05). (D) Co-expression networks constructed with the protein-coding genes, transposable elements, and lncRNAs contained two anti-correlated modules. The two modules may regulate the two cellular states of EPI cells in EB-MB stages and LB-HB stages, respectively. Red and green edges indicated that the two nodes were transcriptionally correlated and anti-correlated, respectively. All data were obtained from 90 batch 2 EPI cells (EB, 29 cells; MB, 20 cells; LB, 24 cells; and HB, 17 cells).
