Lineage segregation in rhesus monkey early embryos. (A) Representative photos of rhesus monkey embryos at the stages of 16-cell (16C), early morula (EM), late morula (LM), early blastocyst (EB), middle blastocyst (MB), late blastocyst (LB), and hatched blastocyst (HB). Dashed lines depict the blastocyst cavities. (B) Lineage segregation path constructed by Monocle based on most variable genes. Five cell states and three lineages were identified. (C) Expression pattern of known marker genes of TE (GATA2, GATA3), PrE (GATA4, SOX17), and EPI (SOX2, NANOG) across the pseudotime assigned by Monocle. (D) Hierarchical clustering based on conserved lineage marker genes identified three cell lineages and fate-undetermined cells.
