DFE for incoming mutations at ESE sites, using different motif sets. Ancestral CpG-filtering was used in all cases. (Blue zone) Effective neutrality; (light yellow zone) very weak negative selection; (dark yellow zone) weak to strong negative selection. Note that although the distribution obtained for RESCUE looks different from that obtained for INT3 or ESR, all three convey very similar information (the majority of mutations within effective neutrality and a minority within strong negative selection). The visual difference is due to the fact that for RESCUE, the best fit was a beta distribution, whereas for the other two it was a two-spikes distribution.
